Neuroprotective effects of polyacrylic acid (PAA) conjugated cerium oxide against hydrogen peroxide- and 6-OHDA-induced SH-SY5Y cell damage

Cerium oxide nanoparticles have been widely investigated against neurodegenerative diseases due to their antioxidant properties that aid in quenching reactive oxygen species. In this study, polyacrylic acid conjugated cerium oxide (PAA-CeO) nanoparticles were synthesized in a 50–60 nm size range with a zeta potential of − 35 mV. X-ray photoelectron spectroscopy analysis revealed a mixed valence state of Ce4+ and Ce3+. PAA-CeO nanoparticles were safe for undifferentiated (UN-) and retinoic acid-differentiated (RA-) human neuroblastoma SH-SY5Y cells and reduced the extent of cell damage evoked by hydrogen peroxide (H2O2) and 6-hydroxydopamine (6-OHDA). In the H2O2 model of cell damage PAA-CeO did not affect the caspase-3 activity (apoptosis marker) but attenuated the number of propidium iodide-positive cells (necrosis marker). In the 6-OHDA model, nanoparticles profoundly reduced necrotic changes and partially attenuated caspase-3 activity. However, we did not observe any impact of PAA-CeO on intracellular ROS formation induced by H2O2. Further, the flow cytometry analysis of fluorescein isothiocyanate-labeled PAA-CeO revealed a time- and concentration-dependent cellular uptake of nanoparticles. The results point to the neuroprotective potential of PAA-CeO nanoparticles against neuronal cell damage induced by H2O2 and 6-OHDA, which are in both models associated with the inhibition of necrotic processes and the model-dependent attenuation of activity of executor apoptotic protease, caspase-3 (6-OHDA model) but not with the direct inhibition of ROS (H2O2 model).

Increased oxidative stress being a result of reactive oxygen species (ROS) overproduction and/or inefficiency of the intracellular antioxidant defense system, is one of the main causes of the development and progression of several neurodegenerative diseases (ND), including the most common Alzheimer's disease (AD) and Parkinson's disease (PD) 1 .ROS are extremely detrimental to cells by disrupting their homeostasis and structural integrity.They could evoke lipid peroxidation, mitochondrial dysfunction, impairments in calcium signaling, and protein tyrosine nitration leading to neuronal cell death through apoptotic or non-apoptotic mechanisms 2,3 .
The excessive intracellular production of hydrogen peroxide (H 2 O 2 ) is a well-known endogenous source of oxidative stress and is widely used to induce oxidative stress in different cell types, such as neuronal cells, cardiomyocytes, retinal epithelial cells and endothelial cells 4,5 .While the neurotoxicity of 6-OHDA results from its ability to generate quinones and reactive oxygen species and it is a widely accepted neurotoxin to model PD under in vitro and in vivo settings 6 .It is mainly taken by dopaminergic or noradrenergic neurons through their transporters and evokes cytotoxic effects by blockade of the mitochondrial respiratory chain complex I or formation of H 2 O 2 , which together lead to excessive intracellular oxidative stress 7 .Under normal conditions, free radicals or ROS are removed by various endogenous enzymatic and nonenzymatic mechanisms to promote cell survival.However, under oxidative stress conditions, a decreased expression and activities of antioxidative

Synthesis of PAA-CeO nanoparticles
The PAA-stabilized CeO 2 nanoparticles were synthesized by low-temperature precipitation method as described previously 18 .A mixed solution containing 30 mM cerium (III) nitrate and ammonium cerium (IV) nitrate with 10% by weight of PAA were prepared and 30% ammonium hydroxide solution was added to this in dropwise manner.After continuous stirring for 24 h, the resultant solution was centrifuged at 4000 rpm for 30 min to separate out large agglomerates.The supernatant was collected and purified by dialyzing against 5 L of water at pH 7 for 2 days.The resulting solution was concentrated, sterile filtered through 20 μm sterile syringe prior to cell culture treatment.Stock solution containing PAA-CeO nanoparticles synthesized with 30 mM cerium (III) nitrate was stored in darkness at room temperature.Additionally, before the experiment it was diluted two (15 mM) and four times (7.5 mM).The final proportion of PAA-CeO nanoparticles added in the cell culture was 10% v/v stock solutions.That was denoted as 0.75 mM, 1.5 mM and 3 mM PAA-CeO.

Characterization
The hydrodynamic particle size and zeta potential of PAA-CeO nanoparticles were determined by Dynamic Light Scattering (DLS) (Zetasizer Nano Series, Malvern Instruments).The sample was measured at 25 °C in triplicate with at least 20 measurements using water as dispersant with parameters set for cerium oxide (refractive index = 2.2 and absorption = 0.001).The particle size and morphology were visualized using Scanning Electron Microscopy (JEOL JSM-7500F, JEOL Ltd.).The X-ray Photoelectron Spectroscopy (XPS) measurements were carried out in a multi-chamber UHV system equipped with a hemispherical analyzer (SES R4000, Gammadata Scienta, Sweden) and non-monochromatized Al Kα (1486.6 eV) X-ray source.The anode was operating at 12 kV and 15 mA.Numerical analysis of the obtained spectra was carried out using CasaXPS 2.3.23 software after

Cell culture
Human neuroblastoma SH-SY5Y cells (ATCC CRL-2266, USA) were cultured in DMEM supplemented with a 10% v/v FBS and 1% v/v penicillin/streptomycin mixture as described previously 25 .Cells were maintained at 37 °C in a saturated, humid atmosphere containing 95% air and 5% CO 2 .After reaching 80% confluency, cells were counted manually (Bürker chamber) and seeded at a density of 4 × 10 4 , 2 × 10 5 and 1 × 10 6 cells per well into 96-, 24-and 6-well plates, respectively.To obtain differentiated cells (RA-SH-SY5Y), the cells were plated at a ratio of half the densities specified for undifferentiated cells and cultured in a medium supplemented with retinoic acid (RA, 10 μM) for 6 days, during which the culture medium was changed every 2 days.One day before cell treatment, the culture medium in both cell phenotypes was exchanged with DMEM containing antibiotics and 1% FBS.The cells were tested for potential Mycoplasma contamination (MycoBlue Mycoplasma Detector, VAZYME) each time the new batch of frozen cells was propagated.The cells were used for experiments between passages 5-15.

Cell treatment
H 2 O 2 and 6-OHDA were prepared freshly in distilled water and were added in volume 1% v/v.In similar volume (1% v/v) were added NAC (1 mM) or Ac-DEVD-CHO (20 µM), which 100 × stock solutions were prepared in DMSO and distilled water, respectively, and stored at − 20 °C.All experimental groups without nanoparticles were supplemented with vehicle (distilled water, 10% v/v).All agents were added to the culture medium at prescribed concentrations under light-limited conditions.
First, UN-and RA-SH-SY5Y cells were treated with PAA-CeO at different concentrations (0.75 mM, 1.5 mM and 3 mM) for 24 h to assess the biosafety of the nanoparticles.Next, to test the neuroprotective potential of developed nanoparticles, the cells were pretreated with PAA-CeO (0.75 mM, 1.5 mM and 3 mM) or vehicle for 30 min followed by 24 h exposure to H 2 O 2 (at IC 50 concentration of 0.375 and 0.5 mM for UN-and RA-SH-SY5Y, respectively) and 6-OHDA (at IC 50 concentration of 0.1 and 0.2 mM for UN-and RA-SH-SY5Y, respectively).The optimal concentration of H 2 O 2 was experimentally established by 24 h treatment of cells with H 2 O 2 at concentrations 0.2-0.5 and 0.2-0.75 mM for UN-and RA-SHSY5Y cells, respectively.The optimal concentrations for 6-OHDA to induce cell damage in UN-and RA-SH-SY5Y cells were established in our previous work 26,27 .As a positive control for both oxidative stress models, an antioxidant N-acetyl cysteine (NAC, 1 mM) was employed, which was given concomitantly with oxidative stress inducers.

Cell viability assay
Cell viability of UN-and RA-SH-SY5Y cells cultured and treated in 96-well plate format was estimated by biochemical WST-1 assay (Roche Diagnostic, Basel, Switzerland) as described previously 25 .The absorbance of each sample was measured 30 and 60 min after substrate addition with a multi-well plate-reader (Infinite® M200 PRO, Tecan, Switzerland) at 440 nM (measurement wavelength) and 630 nm (reference wavelength).Data (calculated difference between measurement and reference measurement) after subtraction for blank value (total damage, 1% Triton X100 for 15 min) were normalized to the vehicle-treated cells and are expressed as a percentage of the control ± SEM established from 3-4 independent experiments with 3-5 replicates each.

Cytotoxicity assay
The level of lactate dehydrogenase (LDH) released from damaged cells into culture media, a marker of cell death, was measured using Cytotoxicity Detection Kit (Roche) as described previously 26 .Twenty four hours after cell treatment in 96-well plate format, 50 µl of the medium was transferred to a new 96-well plate where LDH reagent was added.After 15 min incubation in the dark at room temperature, the absorbance of each sample was measured with the multi-well plate-reader (Infinite® M200 PRO, Tecan, Switzerland) at 490 nm.After subtraction of blank value (absorbance of medium without cells), the data were normalized to the vehicle-treated cells (100%) and presented as a mean ± SEM established from 6-10 independent experiments with 3-5 replicates each.

Propidium iodide staining and flow cytometry
To confirm the results obtained by the LDH release assay, we employed propidium iodide staining of the UN-and RA-SH-SY5Y cells cultured and treated in a 24-well plate format.Twenty four hours after cell treatment, the cells were collected on ice and stained with propidium iodide (PI, 10 µg/ml in DPBS) as described previously 23 .To obtain a maximal signal in PI staining, the cells were treated with TritonX 100 for 5 min.The cells (1 × 10 4 ) were analyzed in the fluorescence channel for PerCP-Cy5-5-A (red fluorescence) using BD FACS Canto II System and BD FACSDiva™ v5.0.1 Software (BD Biosciences, San Jose, CA, USA).The PI-positive cells (exhibiting loss of cell membrane integrity) represent necrotic and late apoptotic cells.Data are presented as a percentage of PI-positive cells (± SEM) established from 2-3 independent experiments with 2 replicates each.

Microscopic assessment of morphological changes
For confirmation of biochemical results on protection mediated by PAA-CeO in UN-and RA-SH-SY5Y cells, 24 h after treatment, the cells were placed in FluoroBrite™ DMEM and were visualized by inverted fluorescence microscope (AxioObserver, Carl Zeiss).The microscopic evaluation was performed using DIC (differential interference contrast) technique and microphotographs were taken using a black-white camera (Axio-CamMRm, Carl Zeiss).www.nature.com/scientificreports/

Caspase-3 activity assay
To assess the impact of tested nanoparticles on apoptotic changes induced by H 2 O 2 or 6-OHDA, we measured the activity of caspase-3, which is a cysteine protease and main executor of apoptosis.The UN-and RA-SH-SY5Y cells were grown in the 6-well plate and were pretreated for 30 min with PAA-CeO (3 mM) or caspase-3 inhibitor, Ac-DEVD-CHO (20 µM) followed by 9 or 18 h exposure to H 2 O 2 and 6-OHDA, respectively.After the cell treatment, the medium was removed, and the plates with cells were stored at − 20 °C until we performed the experiment.The refrozen cells were lysed with ice-cold CAB (caspase assay buffer) supplemented with pepstatin A and leupeptin, centrifuged, and caspase-3 activity was measured in cell supernatants (placed in 96-well plates, each probe in duplicates) using fluorogenic substrate Ac-DEVD-AMC (50 µM in CAB) as described previously 27 .The data (averaged from duplicates and expressed as the mean relative fluorescence units, RFU) after blank subtraction (the signal from CAB buffer) were normalized to the protein level (measured by the BCA method) and further calculated as a percent of vehicle-treated cells and are presented as the mean ± SEM from 2-4 independent experiments with 2 replicates each.

Estimation of ROS formation
Free radical production in UN-SH-SY5Y cells was measured with 5-(and-6)-chloromethyl-2′,7′dichlorodihydrofluorescein diacetate acetyl ester (CM-H2DCFDA, Molecular Probes, USA) as described previously 28 .Briefly, cells cultured in 24-well plate format were loaded with 5 μM CM-H2DCFDA (in Fluoro-Brite™ DMEM).Next, the cells were treated with PAA-CeO (0.75-3 mM) for 15 min and exposed for the next 45 min to 0.5 mM H 2 O 2 .The antioxidant NAC (1 mM) was used as a positive control for the assay.Next, the cells were washed with pre-warmed FluoroBrite™ DMEM, collected in 1.5 ml tubes, centrifuged, and the cell pellet was resuspended in cold DPBS (without calcium and magnesium). 1 × 10 4 cells were analyzed using BD FACS Canto II System and BD FACSDiva™ v5.0.1 Software (BD Biosciences, San Jose, CA, USA) in the fluorescence channel for FITC (green fluorescence).Mean fluorescence intensity was recorded for each sample.Data are presented as mean fluorescent intensity (± SEM) established from 2 independent experiments with 4 replicates each.

Fluorescein isothiocyanate (FITC) labeling of PAA-CeO nanoparticles
For fluorescent labeling of PAA-CeO nanoparticles, 10 µM fluorescein isothiocyanate (FITC) solution in ethanol was suspended in 30 mM PAA-CeO and shaken in the dark at 300 rpm at room temperature overnight (Eppen-dorf® Thermomixer®).Particles were separated via centrifugation at 5000 rpm and washed twice with sterile double-distilled H 2 O until the supernatant turned colorless.The absorption spectra of PAA-CeO, FITC alone and FITC-PAA-CeO were measured using UV-Vis Spectrometer (Shimadzu Corporation) in the wavelength range of 200 to 800 nm.

In vitro cellular uptake of FITC-labeled nanoparticles
To evaluate the cellular uptake of nanoparticles, cells were seeded in 24-well plates.FITC-PAA-CeO (10% v/v) at different concentrations (1.5 mM and 3 mM) were added to UN-and RA-SH-SY5Y cells for 30 min, 1 h, 2 h, 3 h and 6 h.Control cells were treated with a vehicle.After cell treatment, the cells were washed with pre-warmed FluoroBrite™ DMEM and imaged with an inverted fluorescence microscope (AxioObserver, Carl Zeiss) equipped with a black-white camera (Axio-CamMRm, Carl Zeiss).Two microphotographs (in 480 nm and DIC panels) were taken for each well.Next, the cells were collected on ice in 1.5 ml tubes, centrifuged, and the cell pellet was resuspended in cold DPBS (without calcium and magnesium). 1 × 10 4 cells were analyzed using BD FACS Canto II System and BD FACSDiva™ v5.0.1 Software (BD Biosciences, San Jose, CA, USA) in the fluorescence channel for FITC (green fluorescence).Mean fluorescence intensity and percentage of FITC-positive cells were recorded for each sample.Data are presented as a percentage of FITC-positive cells and mean fluorescent intensity (± SEM) established from 2 independent experiments with 2 replicates each.

Statistical analysis
The data were analyzed with Statistica 13.3 software (StatSoft Inc., Tulsa, OK, USA).One-or two-way analysis of variance (ANOVA) with post hoc Duncan's test for multiple comparisons with assumed P < 0.05 were used.

Physiochemical characteristics of synthesized PAA-CeO nanoparticles
PAA conjugated CeO 2 nanoparticles were synthesized by low-temperature chemical precipitation technique.DLS data (Fig. 1a) indicated that PAA-CeO nanoparticles had narrow size distribution with particle size in the range of 50-60 nm and a polydispersity index of 0.258.Negative zeta potential of nanoparticles (− 35.0 mV) indicated their electrostatic stabilization in the aqueous phase (Fig. 1b).The stability of the nanoparticles was ensured by checking the particle size and zeta potential at regular intervals and the values displayed no statistically significant difference even after 3-months of storage (Fig. S1).The spherical morphologies of the samples were visualized using FESEM, which revealed that the average particle size is in line with DLS results (Fig. 1c).
The XPS studies revealed details on the chemical composition, chemical bonds present on the surface and the levels of oxidation of specific components.The high-resolution spectra of C 1 s, Ce 3d and O 1 s (Fig. 2) were used to identify the chemical states of the active phase in the PAA-CeO samples.Three peaks at 285.0 eV (organic contaminants), 286.0-286.6 eV (C-O groups) and 288.8-289.4eV (O-C=O groups) can be identified in the C 1 s spectra (Fig. 2a).The O 1 s spectra (Fig. 2b) exhibit two major contributions (over 80%) located at 529.4 eV and 531.4-531.7 eV related to oxygen in Ce 4+ and Ce 3+ species, respectively.It is worth noting that hydroxyl groups also contribute to the component with O 1 s binding energy close to 531.5 eV.The Ce 3d spectra are presented www.nature.com/scientificreports/ in Fig. 2c.In accordance with the standard approach, Ce 3d spectra can be fitted with eight or ten components marked 'v' for Ce 3d 5/2 and 'u' for Ce 3d 3/2 multiplets.The multiplicity of these states comes from various Ce 4f.level occupancies in the final state.In this case, as it was difficult to resolve the v 0 and u 0 peaks due to the short energy gap between the v and u peaks, we have decided to employ an 8-components technique.The peaks at 882.5 (v), 888.1 (v″) and 898.8 eV (v′′′) as well as 901.1 (u), 906.8 (u") and 917.0 eV (u′′′) can be assigned to Ce 4+ , whereas, 885.8 (v′) and 903.9 eV (u′) to Ce 3+ .The position of Ce 4+ reference line (u′′′) was 917.0 eV, in excellent agreement with the data in the literature 29 .The surface concentration of Ce 3+ was determined from the Ce 3+ / (Ce 3+ + Ce 4+ ) peak areas ratio.The content of Ce 3+ found in PAA-CeO was 21.1%.Most likely, the small amount of cerium on the surface makes it more available for exposure to oxygen and hence Ce species are more oxidized.

Biosafety evaluation of PAA-CeO
Twenty four hours of treatment with PAA-CeO nanoparticles in the concentration range of 0.75-3 mM did not evoke any detrimental effect on UN-or RA-SH-SY5Y cells in comparison with vehicle-treated cells as was measured by LDH release assay.The LDH biochemical data were also confirmed by propidium iodide (PI) staining of cells treated for 24 h with 3 mM PAA-CeO and flow cytometry analysis, where no significant changes were observed in the number of PI-positive cells when compared to the vehicle-treated group (Table 1).Since we observed the unspecific interaction between tested nanoparticles and WST-1 assay (data not shown), we excluded this method from further studies, at least from experimental sets where PAA-CeO nanoparticles were tested.

Neuroprotective effects of PAA-CeO nanoparticles against H 2 O 2 -and 6-OHDA-induced cell damage
Before testing the neuroprotective potential of synthesized nanoparticles, the reliable cell damage models were established in UN-and RA-SH-SY5Y cells using cell viability (WST-1) and toxicity (LDH release) assays (Fig. S1 and Table S1).These data confirmed our previous findings 26,27 that concentrations of 0.375 and 0.5 mM of H 2 O 2 for UN-and RA-H-SY5Y cells, respectively, and 0.1 and 0.2 mM 6-OHDA for UN-or RA-SH-SY5Y cells, respectively for 24 h evoke about 50% decrease in cell viability which could be prevented by co-treatment with antioxidant NAC.
In order to test the neuroprotective potential of PAA-CeO, the UN-and RA-SH-SY5Y cells were pretreated with different concentrations of nanoparticles (0.75, 1.5 and 3 mM) for 30 min followed by 24 h exposure to cell damaging factors (H 2 O 2 or 6-OHDA).We observed an almost threefold increase in cytotoxicity after incubation of UN-SH-SY5Y cells with H 2 O 2 (0.375 mM) when compared to vehicle-treated cells, which was substantially reduced by all tested concentrations of PAA-CeO (0.75, 1.5 and 3 mM) (Fig. 3a).Similarly, in RA-SH-SY5Y cells H 2 O 2 (0.5 mM) evoked over threefold increase in LDH release which was significantly attenuated by all tested concentration of PAA-CeO (Fig. 3b).In the model of 6-OHDA-induced cell damage in UN-SH-SY5Y we observed over twofold increase in LDH release after exposure to 0.1 mM 6-OHDA which was substantially reduced by all tested concentration of PAA-CeO (Fig. 3c).Similarly, an almost 2.5-fold increase of cytotoxicity was observed in RA-SH-SY5Y cells incubated for 24 h with 0.2 mM 6-OHDA, which was also significantly reduced by all tested concentrations of PAA-CeO (Fig. 3d).Further, the neuroprotective potential of PAA-CeO was confirmed by PI staining in both cell phenotypes.We found that PAA-CeO at various concentrations partially attenuated the number of damaged (PI-positive) nuclei induced by H 2 O 2 (Fig. 4a,b) or 6-OHDA (Fig. 4c,d).Moreover, protection mediated by PAA-CeO nanoparticles in both cell damage models in UN-and RA-SH-SY5Y cells were also evidenced by light microscopy (DIC imaging) (Figs. 5, 6).

The effect of PAA-CeO on caspase-3 activity in UN-and RA-SH-SY5Y cells
Based on our previous studies which showed the participation of apoptosis measured by caspase-3 activation in the mechanism of H 2 O 2 or 6-OHDA-evoked cell damage in UN-and RA-SH-SY5Y cells 27 , we tested the effect of PAA-CeO on this apoptotic cell death marker.We observed activation of caspase-3 in both models of cell damage (after 9 and 18 h of treatment with H 2 O 2 and 6-OHDA, respectively) in UN-and RA-SH-SY5Y cells which was totally prevented by caspase-3 inhibitor Ac-DEVD-CHO (Tables 2 and 3).That is in line with our previous reports 25,27 .Although PAA-CeO (3 mM) alone significantly inhibited the basal caspase-3 activity after Table 1.Biosafety of PAA-CeO nanoparticles in UN-and RA-SH-SY5Y cells.The UN-and RA-SH-SY5Y cells were treated with PAA-CeO (0.75-3 mM) or vehicle for 24 h.Cell cytotoxicity was measured in a culture medium with LDH release assay and in the cells by propidium iodide (PI) staining.The LDH data were normalized to the vehicle-treated cells, whereas PI data are shown as a percentage of PI-positive cells.The data were analyzed by one-way ANOVA followed by Duncan's posthoc test and are presented as the mean ± SEM from 4-8 independent experiments.n.d.-not determined.

Effects of PAA-CeO on the H 2 O 2 -induced ROS formation in UN-SH-SY5Y cells
Based on our previous data, which demonstrated increased intracellular ROS in the cell damaging effect of H 2 O 2 in SH-SY5Y cells 28 we tested the effect of PAA-CeO on this parameter using fluorescent intracellular ROS probe CM-H2DCFDA.Similar to our previous study, we found about a threefold increase in CM-DCF fluorescence after H 2 O 2 (0.5 mM for 45 min) when compared with the vehicle-treated cells 28 , which was entirely reduced by antioxidant NAC (1 mM) but not changed by PAA-CeO at all tested concentrations (Fig. 7).

Nanoparticle labeling with FITC
Flow cytometry enables the quantification of cell uptake of nanoparticles with their light scattering and fluorescence signals.Since pure PAA-CeO NPs are not fluorescent in nature, it was necessary to conjugate a fluorescent label to impart luminescence.Therefore, a standard fluorescence labeling molecule, FITC, was conjugated with the nanoparticle.Figure 8 shows the absorption spectra of PAA-CeO alone and FITC labeled PAA-CeO compared to pure FITC.Fluorescein isothiocyanate absorbs most efficiently at its peak or excitation maximum of 490 nm, similar to previous literature reports 30 .The absorbance peak was observed at the wavelength of 300 nm, corresponding to the characteristic absorption peak of Ce 4+ of CeO 2 nanoparticles 31 .While FITC labeled PAA-CeO exhibited bands corresponding to FITC around 490 nm as well as for Ce 4+ around 302 nm.Thus, the absorption spectrum depicts the successful loading of FITC in PAA-CeO.

Cell uptake of FITC labeled PAA-CeO nanoparticles
Nanoparticle cellular uptake refers to their ability to penetrate the cell membrane for which we used FITClabeled PAA-CeO at different concentrations (1.5 and 3 mM) that were added to cells for up to 6 h.In vitro cellular uptake of FITC-PAA-CeO nanoparticles was performed using an inverted fluorescence microscope for visual representation and qualitative analysis.We observed a time-and concentration-dependent increase in fluorescence signal in both cell phenotypes (Fig. 9).The flow cytometry analysis of FITC-labeled PAA-CeO demonstrated that the number of FITC positive cells was increased as a function of time and the signals were dependent on their concentration as well.After 6 h of cell incubation with stained nanoparticles, approx.97% and 84% of UN-SH-SY5Y cells were FITC-positive at concentrations of 1.5 and 3 mM, respectively, which was not distinctly different from the result after 6 h of incubation in RA-SH-SY5Y cells (Fig. 10a,b).Additionally, the uptake of FITC-PAA-CeO was at the level of median fluorescence intensity of the FITC signal where 3 mM FITC-PAA-CeO unveiled maximum mean fluorescence intensity after 3 h incubation in UN-SH-SY5Y cells.In contrast, in RA-SH-SY5Y cells, 3 mM exhibited an increasing trend following the treatment and the maximum value was attained at 6 h (Fig. 10c,d).These data point to similar internalization of FITC-PAA-CeO into both cell phenotypes, which depends on the concentration of tested nanoparticles.

Discussion
Most neurodegenerative diseases are associated with increased oxidative stress in the brain 1 .Based on the prominent behavior of cerium oxide as a regenerative antioxidant 12 , it was justified to study the neuroprotective effects of PAA-CeO nanoparticles against cell damage induced by H 2 O 2 and 6-OHDA which, reported previously, mechanistically induce elevated oxidative stress which is detrimental to neuronal cells [25][26][27][28] .Due to their poor water solubility, low targeting effectiveness and toxicity concerns, the applicability of inorganic nanoparticles such as cerium oxide in its pure form is often considered to be limited 32 .Without the conjugation of additional molecular moiety, these nanoparticles usually exhibit a nonselective distribution across the body and exhibit poor colloidal stability.Therefore, their surface properties need to be modified by conjugating specific surface groups, polymers, or biomolecules to overcome these shortcomings 33,34 .In this study, we have successfully synthesized PAA-CeO nanoparticles in the 50-60 nm range with a zeta potential in water of − 35 mV.The effectiveness of the nanoparticle system is also associated with its stability 35 .We observed that the developed PAA-CeO nanoparticle possessed excellent stability without aggregation for up to 3 months (Fig. S1), making them suitable for  www.nature.com/scientificreports/biomedical applications.In the PAA-CeO nanoparticles, cerium oxide exists in mixed oxidation states of Ce 3+ and Ce 4+ , as evidenced by XPS spectra (Fig. 2).The coexistence of two oxidation states enables redox reactions where Ce 3+ is oxidized to Ce 4+ and H 2 O 2 is formed when nanoceria reduces superoxide radicals 36 .Ce 4+ can also oxidize H 2 O 2 to O 2 and regenerate Ce 3+37 .That is a useful way of regenerating reduced nanoceria and removing ROS, which could be beneficial for neuroprotective effects, especially in the models engaging oxidative stress   18 .In an effort to lessen the aberrant generation of ROS resulting from mitochondrial dysfunction, Kwon et al. synthesized triphenylphosphonium-conjugated ceria nanoparticles (TPP-ceria NPs) that were safe for cells and attenuated mitochondrial ROS 41 .However, in our study, we excluded the direct effect of PAA-CeO on intracellular ROS production (Fig. 7), suggesting an involvement of other intracellular mechanisms in the neuroprotective effects of these polymer-conjugated nanoparticles.The capability of nanoparticles to penetrate the cell membrane is often referred to as cellular uptake.It was necessary to do an additional labeling step since unmodified PAA-CeO are not fluorescent.Hence, a standard fluorescence labeling molecule, FITC, was consequently adsorbed onto the surface of the nanoparticles.A similar conjugation technique has been applied earlier by Jochums et al. to investigate the uptake of titanium oxide nanoparticles in human lung carcinoma (A549) and mouse fibroblast (NIH/3T3) cells 42 .Our results showed the relatively fast cellular uptake of FITC-PAA-CeO in SH-SY5Y cells, which was rather not affected by the differentiation state of the cells.These data extend the usefulness of SH-SY5Y cells as a neuronal-like model for studies on neuroprotective potency not only for polyelectrolyte-coated nanocapsules 43 but also for polymer-conjugated cerium oxide nanoparticles.
Most research reports have demonstrated the evident utility of cerium oxide systems in neurodegenerative diseases at therapeutic doses, in which cerium oxide did not exhibit any toxicity at a wide range of concentration [18][19][20][21][22] .We showed for the first time that PAA-CeO is neuroprotective against H 2 O 2 -and 6-OHDA-induced cell damage in neuronal cells, and this effect was associated with the inhibition of necrosis in both tested models of cell damage (H 2 O 2 and 6-OHDA).Since in our experimental model, during the cell treatment, we used a cell culture medium with 1% FBS, which could slow down the cell proliferation and sensitize cells (serum deprivation) to harmful stimuli, it is not excluded that necrotic processes predominated.Previous experimental data on cerium oxide-mediated brain protection implicated the inhibition of apoptosis among mechanisms of its beneficial effects 20,21 .Since we used models of neuronal cell damage models that engage mixed apoptotic-necrotic mechanisms 26,27 , we attempted to verify how PAA-CeO affects the H 2 O 2 -and 6-OHDA -stimulated caspase-3 activity.In both models of cell damage (H 2 O 2 and 6-OHDA), we showed participation of necrosis and apoptosis when their contribution to cell death probably is not equal.Thus in H 2 O 2 model, when probably necrosis predominates, we did not find any effect of PAA-CeO on caspase-3 activity, whereas in 6-OHDA model we showed partial reduction of this enzyme activity by tested nanoparticles.These data rather suggest a marginal role of the inhibition of caspase-3 as a main mechanistic factor in the PAA-CeO-mediated neuroprotection, at least in our human neuronal-like model.However, at the basal level of caspase-3 activity, we observed the inhibitory effect of PAA-CeO alone, proving that this compound could attenuate apoptotic changes.Although several studies revealed the protective efficacy of cerium oxide systems against oxidative damage in vitro, the mechanism of protection between apoptotic and necrotic cell death is still unknown.Most reported studies are focused on neuroprotection against apoptotic cell damage, and no previous studies demonstrated the apoptotic-necrotic mechanisms in cerium oxide systems.Intracellular protection mechanisms against oxidative free radical damage include scavenging enzyme systems, which lower the level of ROS, such as superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx) 44 .Therefore, our future work will rely on these enzymes to better understand how these scavenging enzymes control H 2 O 2 and 6-OHDA-induced cell damage.There is also further potential to study the involvement of other signaling pathways like the activity of calcium-dependent cysteine protease, calpain; caspase-3 independent apoptosis, lysosomal enzymes (e.g., cathepsin D), or intracellular pro-survival pathways like PI3-K/Akt and ERK1/2 [25][26][27] . https://doi.org/10.1038/s41598-023-45318-6

Figure 9 .
Figure 9. Representative images (green and DIC channels) of cellular uptake of FITC-labelled PAA-CeO nanoparticles (1.5 and 3 mM) after incubation for 30 min and 3 h in (a) UN-and (b) RA-SH-SY5Y cells.

Table 2 .
Effects of PAA-CeO on the H 2 O 2 -induced caspase-3 activity in UN-and RA-SH-SY5Y cells.